Explain the CUBE

X - Application

  1. Protein Expression
  2. Cell Proliferation

Y - Process Mode

  1. Continuous cultivation
  2. Cell Retention Perfusion cultivation
  3. Batch or Fed-Batch cultivation

Z – Technology

  1. Reactors
  2. Sensors
  3. Pumps
  4. Process-Control

The CUBE guide us to solutions

X - Application

  1. Protein expression = secretion of molecules such as proteins by micro-organism.
  2. Cell Proliferation = fast unlimited multiplication of identical micro-organism.

Y - Process mode within your interest

  1. Continuous Cultivation = steady state conditions with constant media flow and harvest.
  2. Cell Retention / Perfusion = “protein expression” based on; 1. Sequential harvest of product from micro-organism, 2. Sequential removal of micro-organism, 3. Sequential media exchange.
  3. Batch and Fed-batch = 1. Batch operation to which no fresh media is added and no broth is removed, 2. Fed-batch to which fresh medium is added and no broth is removed before process termination.

Z - Technologies within our processing

  1. Reactors = Stirred-Tank-Reactors, Fixed-Bed-Reactors, Scaffolding-Reactor, Cell-Retention-Reactor, Perfusion-Reactor, Fermenter, Bioreactor - preferably of Single-Use design.
  2. Sensors = for on-line monitoring of process parameters as input to PCS - preferably of Single-Use design.
  3. Pumps = devices for conveying, moving fluids or performing harvest by mechanical means - preferably of Single-Use design.
  4. Process-Control-System = digital computer used for control of processes via sensor input and actuator output.